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KMID : 0903619870280010018
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Journal of the Korean Society for Horticultural Science
1987 Volume.28 No. 1 p.18 ~ p.23
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Factors Affecting Callus Formation and Organogenesis from in vitro Culture of Pea (Pisum sativum L.)
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Abstract
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The effects of auxins, cytokinins, MS salt strength, and casein hydrolysate on regeneration from individual parts of pea(Pisum sativum L.) were investigated by in vitro Culture.
Higher concentrations of BA and NAA were the most influencial on callus formation and low BA and NAA concentrations resulted in root differentiation. Shoot was differentiated only on the medium supplemented with 1.0㎎/ℓ or 5.0㎎/ℓ BA. Callus formation arid shoot differentiation were better on the full strength of MS medium than that of half strength. Especially when nodes were cultured on the full strength medium, higher rate of shoot initiation was observed.
Casein hydrolpsate(CH) added to MS basal medium with 1㎎/ℓ Benzyladenine and 2㎎/ℓ a-Naphthalene acetic acid stimulated callus formation and organogenesis. Adventitious roots were also initiated from hypocotyl cultured on the same medium.
Shoots originated from the callus formed on the MS medium supplemented with 0.2㎎/ℓ NAA and 5.0㎎/ℓ BA were transferred to MS medium supplemented with 5㎎/ℓ NAA and 0.2㎎/ℓ BA for root differentiation, but the frequency of rooting was very low.
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